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International Journal of Clinical & Medical Images

ISSN: 2376-0249 Open Access

Chlamydophila pneumoniae by micro-immunoflurescence

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Fluorescence microscopic observation, for the indirect detection of IgG antibodies against Chlamydophila pneumoniae by micro-immunoflurescence (MIF) considered the gold standard serological. The test uses purified elementary bodies of C pneumoniae as the antigen and fluorescein isothiocyanate (FITC) as the marker compound. When present in patient serum, C pneumoniae antibodies will combine with C pneumoniae antigens, attached to the glass surface of a microscopic slide: the residual patient sample is removed by washing and flurescein conjugated anti-human antibodies are added, the slide is washed and green flurescence is detected microscopically at a magnification of 400x. The serum sample belonged to a patient at the time that he developed acute myocardial infarction, showing a high titer of 1:512.

Corresponding author
Guadalupe Garcia-Elorriaga
Hospital de Infectología